Clone:
REA496
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
MIC11, MSK21, Protectin, MAC-IP, MIRL, G344, HRF-20, HRF20, MACIF

Extended validation for CD59 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA496
p282 (H19)++
MEM-43-
OV9A2-
1F5-
Cells were incubated with an excess of purified unconjugated CD59 (REA496) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD59 (REA496). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD59 (REA496). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD59 (REA496). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD59 Antibody, anti-human, REAfinity™

Overview

Clone REA496 recognizes the human CD59 antigen, a 20 kDa LY-6 like protein, which regulates the action of the complement membrane attack complex on homologous cells. This glycoprotein is widely distributed on the membranes of human erythrocytes and leukocytes. CD59, also known as protectin, was observed in vascular endothelia throughout the body, in extravascular tissues, and was also found in ductal epithelia of pancreatic, biliary and salivary systems, bronchi, and kidney collecting ducts. Furthermore, CD59 is expressed in the epidermis and in the syncytiotrophoblast of placenta.
Additional information: Clone REA496 displays negligible binding to Fc receptors.

Alternative names

MIC11, MSK21, Protectin, MAC-IP, MIRL, G344, HRF-20, HRF20, MACIF

Detailed product information

Technical specifications

CloneREA496
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
chimpanzee (
Pan troglodytes
)
, baboon
AntigenCD59
Alternative names of antigenMIC11, MSK21, Protectin, MAC-IP, MIRL, G344, HRF-20, HRF20, MACIF
Molecular mass of antigen [kDa]9
Distribution of antigenred blood cells, leukocytes
Entrez Gene ID966
RRIDAB_2734070, AB_2751973, AB_2658782, AB_2658783, AB_2658784, AB_2658785, AB_2734069

Resources for CD59 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD59 Antibody, anti-human, REAfinity™

Publications

  1. Davies, A. et al. (1989) CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. J. Exp. Med. 170(3): 637-654
  2. Philbrick, W. M. et al. (1990) The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility. Eur. J. Immunol. 20(1): 87-92
  3. Meri, S. et al. (1991) Distribution of protectin (CD59), a complement membrane attack inhibitor, in normal human tissues. Lab. Invest. 65(5): 532-537

Related products for
CD59 Antibody, anti-human, REAfinity™

2 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?