Clone:
REAL242
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
VLA-6, ITGA6B, VLA-6α, α6 integrin, CD49fB

Extended validation for CD49f Antibody, anti-mouse, REAdye_lease™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD49f. Human peripheral blood mononuclear cells (PBMCs) were stained with CD49f antibodies and with a suitable counterstaining. As a control, CD49f antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49f. Human peripheral blood mononuclear cells (PBMCs) were stained with CD49f antibodies and with a suitable counterstaining. As a control, CD49f antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49f. Human peripheral blood mononuclear cells (PBMCs) were stained with CD49f antibodies and with a suitable counterstaining. As a control, CD49f antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49f. Human peripheral blood mononuclear cells (PBMCs) were stained with CD49f antibodies and with a suitable counterstaining. As a control, CD49f antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49f. Human peripheral blood mononuclear cells (PBMCs) were stained with CD49f antibodies and with a suitable counterstaining. As a control, CD49f antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49f. Human peripheral blood mononuclear cells (PBMCs) were stained with CD49f antibodies and with a suitable counterstaining. As a control, CD49f antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49f. Human peripheral blood mononuclear cells (PBMCs) were stained with CD49f antibodies and with a suitable counterstaining. As a control, CD49f antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD49f. Human peripheral blood mononuclear cells (PBMCs) were stained with CD49f antibodies and with a suitable counterstaining. As a control, CD49f antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD49f (REAL242). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD49f (REAL242). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD49f (REAL242). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD49f Antibody, anti-mouse, REAdye_lease™

Overview

Clone REAL242 is an antibody fragment derived from the full CD49f antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL242 recognizes the CD49f antigen, a 120kD Single-pass type I membrane protein which is also known as Integrin alpha-6 (ITGA6). Integrins are integral cell-surface proteins composed of an α chain and a β chain and are involved in cell adhesion as well as cell-surface mediated signaling. CD49f associates with integrin β1 chain (CD29) to form VLA-6 and with integrin β4 chain (CD104) to form the α6 β4 complex, which plays a critical structural role in the hemidesmosome and constitutes a receptor for laminin in epithelial cells. CD49f is furthermore expressed on T cells, monocytes, platelets, endothelial cells, trophoblasts of placenta, and spermatogonial stem cells.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

VLA-6, ITGA6B, VLA-6α, α6 integrin, CD49fB

Detailed product information

Technical specifications

CloneREAL242
Clonalitymonoclonal
Isotype controlControl Antibody
Hosthuman cell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD49f
Alternative names of antigenVLA-6, ITGA6B, VLA-6α, α6 integrin, CD49fB
Distribution of antigenepithelial cells, lymphocytes, megakaryocytes, monocytes, platelets, T cells, stem cells, cancer stem cells, hematopoietic stem and progenitor cells, ES and iPS cells, thymocytes, brain, kidney, liver, lung, ovary, spleen

Resources for CD49f Antibody, anti-mouse, REAdye_lease™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD49f Antibody, anti-mouse, REAdye_lease™

Publications

  1. Hemler, M. E. et al. (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures. J. Biol. Chem. 263: 7660-7665
  2. Carter, W. G. et al. (1990) Distinct functions for integrins α 3 β 1 in focal adhesions and α 6 β 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes. J. Cell Biol. 111: 3141-3154
  3. Aumailley, M. et al. (1990) Antibody to integrin α 6 subunit specifically inhibits cell-binding to laminin fragment 8. Exp. Cell Res. 188(1): 55-60
  4. Hynes, R. O. (2002) Integrins: bidirectional, allosteric signaling machines. Cell 110(6): 673-687
  5. Izadyarz, F. et al. (2011) Identification and characterization of repopulating spermatogonial stem cells from the adult human testis. Hum. Reprod. 26(6): 1296-1306

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