Clone:
REA616
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, ADPRC 1, I-19, NIM-R5, T10

Extended validation for CD38 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA616
90.4++
90++
Cells were incubated with an excess of purified unconjugated CD38 (REA616) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD38 Splenocytes from C57BL/6 mice were stained with CD38 antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD38 Splenocytes from C57BL/6 mice were stained with CD38 antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD38 Splenocytes from C57BL/6 mice were stained with CD38 antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD38 Splenocytes from C57BL/6 mice were stained with CD38 antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD38 Splenocytes from C57BL/6 mice were stained with CD38 antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD38 (REA616). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD38 (REA616). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD38 (REA616). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD38 Antibody, anti-mouse, REAfinity™

Overview

Clone REA616 recognizes the mouse CD38 antigen, a 42 kDa single-pass type II membrane protein, which is also known as ADP-ribosyl cyclase 1. CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis. In the mouse, CD38
+
hematopoietic cells include B cells, subsets of T cells, monocytes, and macrophages. CD38 expression on these cells is modulated following activation and differentiation. In contrast to humans, CD38 expression is downregulated on mouse germinal center B cells and mouse mature plasma cells.
Additional information: Clone REA616 displays negligible binding to Fc receptors.

Alternative names

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, ADPRC 1, I-19, NIM-R5, T10

Detailed product information

Technical specifications

CloneREA616
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD38
Alternative names of antigenADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, ADPRC 1, I-19, NIM-R5, T10
Molecular mass of antigen [kDa]34
Distribution of antigenB cells, macrophages, monocytes, T cells
Entrez Gene ID12494
RRIDAB_2802049, AB_2801980, AB_2819754, AB_2819758, AB_2657837, AB_2657838, AB_2657839, AB_2657840, AB_2657845, AB_2657846, AB_2657847, AB_2657848, AB_2811415

References for CD38 Antibody, anti-mouse, REAfinity™

Publications

  1. Oliver, A. M. et al. (1997) Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells. J. Immunol. 158(3): 1108-1115
  2. Cockayne, D. A. et al. (1998) Mice deficient for the ecto-nicotinamide adenine dinucleotide glycohydrolase CD38 exhibit altered humoral immune responses. Blood 92(4): 1324-1333
  3. Howard, M. et al. (1993) Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38. Science 262(5136): 1056-1059
  4. Bean, A. G. et al. (1995)
    CD38 expression on mouse T cells: CD38 defines functionally distinct subsets of alpha beta TCR
    +
    CD4
    -
    CD8
    -
    thymocytes.
    Int. Immunol. 7(2): 213-221

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