CD271 MicroBead Kits have been developed for the positive selection or depletion of cells expressing CD271 (LNGFR). They are ideally suited for the isolation of CD271
+
mesenchymal stromal cells (MSCs) from human tissues, including bone marrow and lipoaspirate. In addition, CD271 MicroBead Kits have been used successfully for the isolation of MSCs from bone marrow of monkey, goat, dog, pig, and sheep.

Data and images for CD271 MicroBead Kits, human

Figures

Figure 1

Enrichment of CD271
+
mesenchymal stromal cells (MSCs) from bone marrow mononuclear cells (BM-MNCs) using the CD271 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD271 antibodies conjugated to PE as well as CD45-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer.
BM-MNCs before separation
CD271
+
cells
View details

Figure 1

Enrichment of CD271
+
mesenchymal stromal cells (MSCs) from bone marrow mononuclear cells (BM-MNCs) using the CD271 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD271 antibodies conjugated to PE as well as CD45-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer.
View details

Figure 1

Enrichment of CD271
+
mesenchymal stromal cells (MSCs) from bone marrow mononuclear cells (BM-MNCs) using the CD271 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD271 antibodies conjugated to PE as well as CD45-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer.

Specifications for CD271 MicroBead Kits, human

Overview

CD271 MicroBead Kits have been developed for the positive selection or depletion of cells expressing CD271 (LNGFR). They are ideally suited for the isolation of CD271
+
mesenchymal stromal cells (MSCs) from human tissues, including bone marrow and lipoaspirate. In addition, CD271 MicroBead Kits have been used successfully for the isolation of MSCs from bone marrow of monkey, goat, dog, pig, and sheep.

Detailed product information

Background information

CD271, also known as LNGFR (low-affinity nerve growth factor receptor) or p75NTR, belongs to the low-affinity neurotrophin receptor and the tumor necrosis factor receptor superfamily.
CD271 is a well-known marker on mesenchymal stem cells, also known as mesenchymal stromal cells (MSCs), from bone marrow aspirate
1–4
or lipoaspirate
5
. After separation colony-forming unit fibroblast (CFU-F) activity was found only in the CD271
+
cell fraction, and not in the CD271
population
6–8
. Isolated CD271
+
cells have a higher proliferative capacity in comparison to MSCs isolated by plastic adherence
6,8,9
. Secretion of growth factors was significantly higher in the separated CD271
+
MSCs
7
.
CD271 (LNGFR) was initially described to be expressed on cells of the central and peripheral nervous system nervous system and was suggested to be involved in the development, survival, and differentiation of neural cells
10
. CD271
+
neural crest progenitor cells can be efficiently isolated with Neural Crest Stem Cell MicroBeads.

Cross-reactivity

The CD271 antibody shows cross-reactivity with monkey, goat, dog, pig, and sheep
11
.

Columns

MS, LS, XS, or autoMACS
®
Columns.

References for CD271 MicroBead Kits, human

Publications

  1. Jones, E.A. et al. (2004) 4th Annual Conference on Mesenchymal and Nonhematopoietic stem cells : abstract
  2. Jones, E.A. et al. (2004) ASH meeting 29(abstract): 2337
  3. Jones et al. (2006) Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow. Cytometry B Clin. Cytom. 70: 391-399
  4. Jones, E.A. et al. (2007) Purification of proliferative and multipotential marrow stromal cells (MSCs) from bone marrow aspirate by selection for CD271 (LNGFR) expression. MACS&more 11(1): 22-25
  5. Meyerrose, T. E. et al. (2007)
    In vivo
    distribution of human adipose-derived mesenchymal stem cells in novel xenotransplantation models.
    Stem Cells 25: 220-227
  6. Quirici, N. et al. (2002) Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies. Exp. Hematol. 30: 783-791
  7. Kuci et al. (2010) CD271 antigen defines a subset of multipotent stromal cells with immunosuppressive and lymphohematopoietic engraftment-promoting properties. Haematologica 95: 651-659
  8. Jarocha, D. et al. (2008)
    Adventage of mesenchymal stem cells (MSC) expansion directly from purified bone marrow CD105
    +
    and CD271
    +
    cells.
    Folia Histochem. Cytobiol. 46: 307-314
  9. Poloni et al. (2009)
    Selection of CD271
    +
    cells and human AB serum allows a large expansion of mesenchymal stromal cells from human bone marrow.
    Cytotherapy 11: 153-162
  10. Thomson, T. M. et al. (1988) Expression of human nerve growth factor receptor on cells derived from all three germ layers. Exp. Cell Res. 174: 533-539
  11. Rozemuller, H. et al. (2010) Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies. Stem Cells Dev. 19: 1911-1921

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