CD207 (Langerin) MicroBeads were developed for the isolation of CD207
+
dendritic cells from single-cell suspensions of human epidermis.

Data and images for CD207 (Langerin) MicroBeads, human

Figures

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were spiked
with CD207-transfected 1881 cells to obtain a 5% initial frequency
of CD207
+
cells. CD207
+
cells were isolated using
CD207 (Langerin) MicroBeads, an MS Column and a MiniMACS™
Separator. Cells were stained with CD207 (Langerin)-PE (# 130-
098-355) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis
based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
View details

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were spiked
with CD207-transfected 1881 cells to obtain a 5% initial frequency
of CD207
+
cells. CD207
+
cells were isolated using
CD207 (Langerin) MicroBeads, an MS Column and a MiniMACS™
Separator. Cells were stained with CD207 (Langerin)-PE (# 130-
098-355) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis
based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were spiked
with CD207-transfected 1881 cells to obtain a 5% initial frequency
of CD207
+
cells. CD207
+
cells were isolated using
CD207 (Langerin) MicroBeads, an MS Column and a MiniMACS™
Separator. Cells were stained with CD207 (Langerin)-PE (# 130-
098-355) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis
based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were spiked
with CD207-transfected 1881 cells to obtain a 5% initial frequency
of CD207
+
cells. CD207
+
cells were isolated using
CD207 (Langerin) MicroBeads, an MS Column and a MiniMACS™
Separator. Cells were stained with CD207 (Langerin)-PE (# 130-
098-355) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis
based on scatter signals and propidium iodide fluorescence.

Specifications for CD207 (Langerin) MicroBeads, human

Overview

CD207 (Langerin) MicroBeads were developed for the isolation of CD207
+
dendritic cells from single-cell suspensions of human epidermis.

Detailed product information

Background information

Langerin (CD207 antigen), a 40 kDa, glycosylated type II transmembrane C-type lectin receptor, is described as the state-ofthe-art marker for identifying Langerhans cells (LCs) in humans and mice
(1)
. LCs are a subset of dendritic cells (DCs) cells that reside in epithelia; the best studied example of these antigen-presenting cells are LCs of the epidermis. Their unique identifying feature are organelles called Birbeck Granules (BGs), which can be detected by electron microscopy. CD207 is expressed at both the surface membrane and in the cytoplasmatic BGs. CD207 plays a role as an endocytic receptor. It is directly involved in antigen capture and its endocytosis and induces the formation of BGs in immature DCs
(2)
.

Detailed separation procedure

Single-cell suspensions of human epidermis are magnetically labeled with CD207 (Langerin) MicroBeads. The cell suspension is loaded onto a MACS
®
Column, in the magnetic field of a MACS Separator. The magnetically labeled cells are retained within the column. Unlabeled cells, depleted of CD207
+
cells, can be collected in the flow through. After removing the column from the magnetic field, CD207
+
cells can be eluted as the positively selected cell fraction.

Columns

MS, LS, or autoMACS
®
Columns.

References for CD207 (Langerin) MicroBeads, human

Publications

  1. Valladeau, J. et al. (2000) Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules. Immunity 12(1): 71-81
  2. Romani, N. et al. (2010) Langerhans cells and more: langerin-expressing dendritic cell subsets in the skin. Immunol. Rev. 234(1): 120-141

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