Clone:
REA817
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
LY75, DEC-205, DEC205

Extended validation for CD205 (DEC205) Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA817
NLDC-145++
H83 (h/m/r)-
V18-949-
205yekta-
NLDC-145++
Cells were incubated with an excess of purified unconjugated CD205 (DEC205) (REA817) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD205 (DEC205). C57BL6/6 splenocytes were stained with CD205 (DEC205) antibodies and with a suitable counterstaining. As a control, CD205 (DEC205) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD205 (DEC205). C57BL6/6 splenocytes were stained with CD205 (DEC205) antibodies and with a suitable counterstaining. As a control, CD205 (DEC205) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD205 (DEC205). C57BL6/6 splenocytes were stained with CD205 (DEC205) antibodies and with a suitable counterstaining. As a control, CD205 (DEC205) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD205 (DEC205). C57BL6/6 splenocytes were stained with CD205 (DEC205) antibodies and with a suitable counterstaining. As a control, CD205 (DEC205) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD205 (DEC205). C57BL6/6 splenocytes were stained with CD205 (DEC205) antibodies and with a suitable counterstaining. As a control, CD205 (DEC205) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD205 (DEC205). C57BL6/6 splenocytes were stained with CD205 (DEC205) antibodies and with a suitable counterstaining. As a control, CD205 (DEC205) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD205 (DEC205). C57BL6/6 splenocytes were stained with CD205 (DEC205) antibodies and with a suitable counterstaining. As a control, CD205 (DEC205) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD205 (DEC205) (REA817). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD205 (DEC205) (REA817). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD205 (DEC205) (REA817). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD205 (DEC205) Antibody, anti-mouse, REAfinity™

Overview

Clone REA817 recognizes the mouse CD205 antigen, a 205 kDa integral membrane glycoprotein also known as DEC205 (dendritic and epithelial cells, 205 kDa). This membrane protein acts as an endocytic receptor and thereby mediates efficient processing and presentation of antigens
in vivo
, leading to the induction of T cell immunity or tolerance. CD205 is expressed at high levels on mouse dendritic cells (DCs) in the skin (Langerhans cells), on DCs residing in the T cell areas of peripheral lymphoid organs, and on DCs generated
in vitro
from bone marrow progenitors. To a much lower extent, CD205 is also expressed on mature B cells, granulocytes, and T cells.
Additional information: Clone REA817 displays negligible binding to Fc receptors.

Alternative names

LY75, DEC-205, DEC205

Detailed product information

Technical specifications

CloneREA817
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD205 (DEC205)
Alternative names of antigenLY75, DEC-205, DEC205
Molecular mass of antigen [kDa]195
Distribution of antigendendritic cells, T cells, Langerhans cells, skin
Entrez Gene ID17076
RRIDAB_2656191, AB_2656192, AB_2656193, AB_2656194, AB_2656195, AB_2656196, AB_2656197, AB_2784061, AB_2784060, AB_2656198, AB_2656199, AB_2656190

Resources for CD205 (DEC205) Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD205 (DEC205) Antibody, anti-mouse, REAfinity™

Publications

  1. Kraal, G. et al. (1986) Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J. Exp. Med. 163: 981-997
  2. Witmer-Pack, M. D. et al. (1995)
    Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. II. Expression
    in situ
    in lymphoid and nonlymphoid tissues.
    Cell. Immunol. 163: 157-162
  3. Inaba, K. et al. (1995) Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes. Cell. Immunol. 163: 148-156
  4. Bonifaz, L. et al. (2002)
    Efficient targeting of protein antigen to the dendritic cell receptor DEC-205 in the steady state leads to antigen presentation on major histocompatibility complex class I products and peripheral CD8
    +
    T cell tolerance.
    J. Exp. Med. 196: 1627-1638

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