Clone:
REA148
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
MSR1, MRS-A, MSR, SR-AI, SR-AII, SCARA1, Scvr, SRA

Extended validation for CD204 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA148
268318++
2F8++
M204PA+
Cells were incubated with an excess of purified unconjugated CD204 (REA148) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD204. Thioglycolate-elicited peritoneal exudate cells from C57BL/6 mice were stained with CD204 antibodies and with a suitable counterstaining. As a control, CD204 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD204. Thioglycolate-elicited peritoneal exudate cells from C57BL/6 mice were stained with CD204 antibodies and with a suitable counterstaining. As a control, CD204 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD204. Thioglycolate-elicited peritoneal exudate cells from C57BL/6 mice were stained with CD204 antibodies and with a suitable counterstaining. As a control, CD204 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD204. Thioglycolate-elicited peritoneal exudate cells from C57BL/6 mice were stained with CD204 antibodies and with a suitable counterstaining. As a control, CD204 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD204. Thioglycolate-elicited peritoneal exudate cells from C57BL/6 mice were stained with CD204 antibodies and with a suitable counterstaining. As a control, CD204 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD204 (REA148). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD204 (REA148). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD204 (REA148). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD204 Antibody, anti-mouse, REAfinity™

Overview

Clone REA148 recognizes mouse CD204, also known as MSR 1 or SRA (murine scavenger receptor class A type I and II). It supports the recognition and ingestion of apoptotic cells and in particular it plays a supporting role in apoptotic cell clearance in embryogenesis. CD204 is mainly expressed on tissue macrophages.
Additional information: Clone REA148 displays negligible binding to Fc receptors.

Alternative names

MSR1, MRS-A, MSR, SR-AI, SR-AII, SCARA1, Scvr, SRA

Detailed product information

Technical specifications

CloneREA148
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD204
Alternative names of antigenMSR1, MRS-A, MSR, SR-AI, SR-AII, SCARA1, Scvr, SRA
Molecular mass of antigen [kDa]50
Distribution of antigenmacrophages
Entrez Gene ID20288
RRIDAB_2784058, AB_2656168, AB_2656169, AB_2656172, AB_2656173, AB_2656174, AB_2656175, AB_2656176, AB_2656177, AB_2656178, AB_2656179, AB_2656180, AB_2656181, AB_2784059

Resources for CD204 Antibody, anti-mouse, REAfinity™

Certificates

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Reviews for CD204 Antibody, anti-mouse, REAfinity™

CD204 Antibody

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CD204-VioBlue, mouse (130-102-195)

Macrophages were assessed for CD204 expression. Miltenyi's anti-CD204 was chosen because of the color options offered and bright, specific staining.

CD204 Antibody

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CD204-VioBlue, mouse (130-103-019)

Macrophages were assessed for CD204 expression. Miltenyi's anti-CD204 was chosen because of the color options offered and bright, specific staining.

References for CD204 Antibody, anti-mouse, REAfinity™

Publications

  1. Komohara, Y. et al. (2005) Clearance of apoptotic cells is not impaired in mouse embryos deficient in class A scavenger receptor types I and II (CD204). Dev. Dyn. 232(1): 67-74

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