Clone:
LT20
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
MS4A1, B1, Bp35, CVID5, LEU-16, MS4A2, S7, Ly-44

Extended validation for CD20 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with LT20
REA780++
2H7++
Hu2++
LT27++
Cells were incubated with an excess of purified unconjugated CD20 (LT20) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD20. Human peripheral blood mononuclear cells (PBMCs) were stained with CD20 antibodies and with a suitable counterstaining. As a control, CD20 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD20. Human peripheral blood mononuclear cells (PBMCs) were stained with CD20 antibodies and with a suitable counterstaining. As a control, CD20 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD20. Human peripheral blood mononuclear cells (PBMCs) were stained with CD20 antibodies and with a suitable counterstaining. As a control, CD20 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD20. Human peripheral blood mononuclear cells (PBMCs) were stained with CD20 antibodies and with a suitable counterstaining. As a control, CD20 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD20. Human peripheral blood mononuclear cells (PBMCs) were stained with CD20 antibodies and with a suitable counterstaining. As a control, CD20 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD20 (LT20). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD20 (LT20). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD20 (LT20). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD20 Antibody, anti-human

Overview

The antibody recognizes the CD20 antigen, a non-glycosylated transmembrane protein of 33–37 kDa that is expressed on B lineage cells from the pre–B cell stage to the B cell lymphoblast stage. The antigen is further expressed on most malignant B cells. CD20 is not found on early B cell progenitors or plasma cells. Oligomers of CD20 form a Ca
2+
channel and might function in the regulation of local responses during B cell activation.

Alternative names

MS4A1, B1, Bp35, CVID5, LEU-16, MS4A2, S7, Ly-44

Detailed product information

Technical specifications

CloneLT20
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD20
Alternative names of antigenMS4A1, B1, Bp35, CVID5, LEU-16, MS4A2, S7, Ly-44
Molecular mass of antigen [kDa]33
Distribution of antigenB cells, dendritic cells, lymphocytes, cancer stem cells
Entrez Gene ID931
RRIDAB_2726142, AB_2726420, AB_2726143, AB_2726417, AB_2726140, AB_2726423, AB_2726146, AB_2726424, AB_2726147, AB_2726421, AB_2726144, AB_2733214, AB_2733215, AB_2733104, AB_2733105, AB_2726422, AB_2726145, AB_2726418, AB_2726141, AB_2732966, AB_2732967, AB_2726419

Resources for CD20 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD20 Antibody, anti-human

Publications

  1. Lamprecht, B. et al. (2008) Aberrant expression of the Tʜ2 cytokine IL-21 in Hodgkin lymphoma cells regulates STAT3 signaling and attracts Treg cells via regulation of MIP-3alpha. Blood 112(8): 3339-3347
  2. Maxwell, S. A. et al. (2009) 14-3-3zeta J. Biol. Chem. 284(33): 22379-22389
  3. Countouriotis, T. B. et al. (2002) Cell surface antigen and molecular targeting in the treatment of hematologic malignancies. Stem Cells 20(3): 215-229
  4. Cragg, M. S. et al. (2002)
    Opposing properties of CD20 mAb; in: Mason, D.
    et al.
    (eds.): Leucocyte typing VII,
    Oxford, Oxford University Press
  5. Polyak, M. J. and Deans, J. P. (2002)
    CD20 Workshop Panel report: in Mason, D.
    et al.
    (eds.): Leucocyte typing VII,
    Oxford, Oxford University Press

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