Clone:
REA245
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
CCR5, CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22, CHEMR13

Extended validation for CD195 (CCR5) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA245
HEK1/85a++
J418F1++
2D7/CCR5-
Cells were incubated with an excess of purified unconjugated CD195 (CCR5) (REA245) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD195 (CCR5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD195 (CCR5) antibodies and with a suitable counterstaining. As a control, CD195 (CCR5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD195 (CCR5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD195 (CCR5) antibodies and with a suitable counterstaining. As a control, CD195 (CCR5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD195 (CCR5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD195 (CCR5) antibodies and with a suitable counterstaining. As a control, CD195 (CCR5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD195 (CCR5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD195 (CCR5) antibodies and with a suitable counterstaining. As a control, CD195 (CCR5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD195 (CCR5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD195 (CCR5) antibodies and with a suitable counterstaining. As a control, CD195 (CCR5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD195 (CCR5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD195 (CCR5) antibodies and with a suitable counterstaining. As a control, CD195 (CCR5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD195 (CCR5) (REA245). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD195 (CCR5) (REA245). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD195 (CCR5) (REA245). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD195 (CCR5) Antibody, anti-human, REAfinity™

Overview

Clone REA245 recognizes the human CD195 antigen, a multi-pass membrane protein which is also known as C-C chemokine receptor type 5 (CCR5). CD195 belongs to the G-protein coupled receptor 1 family and is predominantly expressed on T cells, macrophages, and dendritic cells. It is a receptor for a number of inflammatory CC-chemokines including MIP-1-α, MIP-1-β, and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. CD195 is involved in the chemotaxis of leucocytes to inflammation sites and plays an important role in the recruitment of macrophages, T cells, and monocytes in inflammation. Many forms of HIV initially use CD195 to enter and infect host cells. Additionally, CCR5 may have an indirect effect on cancer progression by controlling the antitumor immune response, since it has been demonstrated that its expression could promote tumor growth and contribute to tumor metastasis.
Additional information: Clone REA245 displays negligible binding to Fc receptors.

Alternative names

CCR5, CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22, CHEMR13

Detailed product information

Technical specifications

CloneREA245
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD195 (CCR5)
Alternative names of antigenCCR5, CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22, CHEMR13
Molecular mass of antigen [kDa]41
Distribution of antigenT cells, macrophages, dendritic cells
Entrez Gene ID1234
RRIDAB_2733783, AB_2752009, AB_2751979, AB_2784040, AB_2784039, AB_2811445, AB_2811439, AB_2801894, AB_2877013, AB_2877014, AB_2876955, AB_2876956, AB_2733782

References for CD195 (CCR5) Antibody, anti-human, REAfinity™

Publications

  1. Li, W. et al. (2016) Proteomics analysis reveals a Tʜ17-prone cell population in presymptomatic graft-versus-host disease. JCI Insight 1(6): e86660
  2. Richardson, M. W. et al. (2012) Kruppel-like factor 2 modulates CCR5 expression and susceptibility to HIV-1 infection. J. Immunol. 189(8): 3815-3821
  3. Shahin, K. et al. (2013) Alterations in chemokine receptor CCR5 expression on blood dendritic cells correlate with acute graft-versus-host disease. Transplantation 96(8): 753-762
  4. Samson, M. et al. (1996) Molecular cloning and functional expression of a new human CC-chemokine receptor gene. Biochemistry 35(11): 3362-3367

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