Clone:
REAL406
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
GHI/61, M130, MM130

Specifications for CD163 Antibody, anti-human, REAdye_lease™

Overview

Clone REAL406 is an antibody fragment derived from the full CD163 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL406 recognizes the human CD163 antigen, a single-chain transmembrane protein also known as hemoglobin scavenger receptor or M130. It is expressed by mature tissue macrophages and peripheral blood monocytes. The expression of CD163 is up-regulated in vitro and in vivo by anti-inflammatory mediators such as IL-10 and (gluco)corticosteroid and is shed to blood upon inflammatory activation of macrophages. CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin. Depending on the ligand, crosslinking of CD163 initiates signal transduction leading to the production of proinflammatory cytokines Il-1ß, IL-6, and GM-CSF or the antiinflammatory cytokine IL-10.
In rheumatoid arthritis, CD163 is used as a marker for the differentiation between synovial macrophages and synovial intimal fibroblasts. During microbial infections and in myelomonocytic leukemias CD163 is expressed in a higher level.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

GHI/61, M130, MM130

Detailed product information

Technical specifications

CloneREAL406
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD163
Alternative names of antigenGHI/61, M130, MM130
Distribution of antigenmacrophages, monocytes, tumor cells, dendritic cells, hematopoietic stem and progenitor cells, tonsil
RRIDAB_2857545

Resources for CD163 Antibody, anti-human, REAdye_lease™

Certificates

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References for CD163 Antibody, anti-human, REAdye_lease™

Publications

  1. Kristiansen, M. et al. (2001) Identification of the haemoglobin scavenger receptor. Nature 409(6817): 198-201
  2. Etzerodt, A. et al. (2019) Specific targeting of CD163(+) TAMs mobilizes inflammatory monocytes and promotes T cell-mediated tumor regression. J. Exp. Med. 216(10): 2394-2411
  3. Shiraishi, D. et al. (2018) CD163 Is Required for Protumoral Activation of Macrophages in Human and Murine Sarcoma. Cancer Res. 78(12): 3255-3266
  4. Ritter, M. et al. (2001) Interaction of CD163 with the regulatory subunit of casein kinase II (CKII) and dependence of CD163 signaling on CKII and protein kinase C. Eur. J. Immunol. 31(4): 999-1009
  5. Burdo, T. H. et al. (2011) Soluble CD163 made by monocyte/macrophages is a novel marker of HIV activity in early and chronic infection prior to and after anti-retroviral therapy. J. Infect. Dis. 204: 154-163
  6. Chao Ni et al. (2019) CD68- and CD163-positive tumor infiltrating macrophages in non-metastatic breast cancer: a retrospective study and meta-analysis. J Cancer 10(19): 4463-4472

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