CD16 MicroBeads have been developed for the isolation of eosinophils by depletion of neutrophils from granulocyte-enriched cell fractions from peripheral blood. Alternatively, neutrophils can be isolated by positive selection. Peripheral blood and various tissues (e.g., bone marrow, nasal tissue) are suitable cell sources.

Data and images for CD16 MicroBeads, human

Figures

Figure 1

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Isolation of eosinophils from peripheral blood using CD16 MicroBeads.

Figure 1

Isolation of eosinophils from peripheral blood using CD16 MicroBeads.

Figure 2

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Eosinophils isolated using CD16 MicroBeads and MACS
®
Technology. (Courtesy of Dr. Hans-Uwe Simon, Davos, Switzerland.)

Figure 2

Eosinophils isolated using CD16 MicroBeads and MACS
®
Technology. (Courtesy of Dr. Hans-Uwe Simon, Davos, Switzerland.)

Isolation of untouched eosinophils from peripheral blood granulocytes using CD16 MicroBeads.

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Cells are flourescently stained with CD16-FITC.
Cells are flourescently stained with CD16-FITC.

Specifications for CD16 MicroBeads, human

Overview

CD16 MicroBeads have been developed for the isolation of eosinophils by depletion of neutrophils from granulocyte-enriched cell fractions from peripheral blood. Alternatively, neutrophils can be isolated by positive selection. Peripheral blood and various tissues (e.g., bone marrow, nasal tissue) are suitable cell sources.

Detailed product information

Background information

The CD16 antigen, which is a low-affinity Fc receptor for aggregated IgG, is expressed on neutrophils, NK cells, and macrophages. CD16 MicroBeads have also been used to deplete NK cells.

Downstream applications

Eosinophils isolated with CD16 MicroBeads are used for the functional analysis of surface molecules
1,3
or for studies on signal transduction
7
, chemotaxis
1,6
, cytokine expression
3,5
, degranulation
4
, and apoptosis
7
.

Columns

LD, CS, D, or autoMACS
®
Columns.

Reviews for CD16 MicroBeads, human

Beads to isolate Fc receptor gamma expressing cells

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CD16 MicroBeads, human (130-045-701)

Our laboratory investigates different ways to improve immune responses against HIV, including enhancement of NK mediated ADCC activity. We use these beads to deplete cells expressing Fc gamma receptor (mostly NK cells) and therefore eliminate ADCC activity as a control in our assays.

CD16 Magentic Beads

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CD16 MicroBeads, human (130-045-701)

CD16 isolation is important for our studies looking at monocyte/macrophage function in vitro. These beads provide an easy and reliable way of isolating this cell population from human PBMCs.

References for CD16 MicroBeads, human

Publications

  1. Blom, M. et al. (1995) Eosinophils isolated with two different methods show different characteristics of activation. J. Immunol. Methods 178: 183-193
  2. Hansel, T. T. et al. (1991) An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils. J. Immunol. Methods 145: 105-110
  3. Levi-Schaffer, F. et al. (1995) Association of granulocyte-macrophage colony-stimulating factor with the crystalloid granules of human eosinophils. Blood 85: 2579-2586
  4. Simon, H. U. et al. (1994) Functional platelet-activating factor receptors are expressed by monocytes and granulocytes but not by resting or activated T and B lymphocytes from normal individuals or patients with asthma. J. Immunol. 153: 364-377
  5. Sriramarao, P. et al. (1996) E-selectin preferentially supports neutrophil but not eosinophil rolling under conditions of flow in vitro and in vivo. J. Immunol. 157: 4672-4680
  6. Motegi, Y. and Kita, H. (1998) Interaction with secretory component stimulates effector functions of human eosinophils but not of neutrophils. J. Immunol. 161: 4340-4346
  7. Yousefi, S. et al. (1996) Requirement of Lyn and Syk tyrosine kinases for the prevention of apoptosis by cytokines in human eosinophils. J. Exp. Med. 183: 1407-1414

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