Clone:
REA363
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
PDGFRB, IBGC4, IMF1, JTK12, Pdgfr, PDGFR-1

Extended validation for CD140b Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA363
28D4++
18A2++
Cells were incubated with an excess of purified unconjugated CD140b (REA363) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD140b. BJ foreskin fibroblasts were stained with CD140b antibodies and with a suitable counterstaining. As a control, CD140b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD140b. BJ foreskin fibroblasts were stained with CD140b antibodies and with a suitable counterstaining. As a control, CD140b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD140b. BJ foreskin fibroblasts were stained with CD140b antibodies and with a suitable counterstaining. As a control, CD140b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD140b. BJ foreskin fibroblasts were stained with CD140b antibodies and with a suitable counterstaining. As a control, CD140b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD140b. BJ foreskin fibroblasts were stained with CD140b antibodies and with a suitable counterstaining. As a control, CD140b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD140b (REA363). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD140b (REA363). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD140b (REA363). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD140b Antibody, anti-human, REAfinity™

Overview

Clone REA363 recognizes the human CD140b antigen, a single-pass type I membrane protein, also known as platelet-derived growth factor receptor beta (PDGFR-β). CD140b is broadly expressed in embryonic tissue and in cells of mesodermal origin in adult tissue as fibroblasts, smooth muscle cells, glial cells, and chondrocytes. It is a tyrosine-protein kinase that acts as a cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration.
Additional information: Clone REA363 displays negligible binding to Fc receptors.

Alternative names

PDGFRB, IBGC4, IMF1, JTK12, Pdgfr, PDGFR-1

Detailed product information

Technical specifications

CloneREA363
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD140b
Alternative names of antigenPDGFRB, IBGC4, IMF1, JTK12, Pdgfr, PDGFR-1
Molecular mass of antigen [kDa]121
Distribution of antigenfibroblasts, chondrocytes, microglia, smooth muscle
Entrez Gene ID5159
RRIDAB_2783952, AB_2783955, AB_2783954, AB_2819556, AB_2819521, AB_2655086, AB_2655087, AB_2655088, AB_2655089, AB_2783953

Resources for CD140b Antibody, anti-human, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD140b Antibody, anti-human, REAfinity™

Publications

  1. Gronwald, R. G. et al. (1988) Cloning and expression of a cDNA coding for the human platelet-derived growth factor receptor: evidence for more than one receptor class. Proc. Natl. Acad. Sci. U.S.A. 85(10): 3435-3439
  2. Jin, S. et al. (2008) Notch signaling regulates platelet-derived growth factor receptor-beta expression in vascular smooth muscle cells. Circ. Res. 102(12): 1483-1491
  3. Ball, S. G. et al. (2012) Inhibition of platelet-derived growth factor receptor signaling regulates Oct4 and Nanog expression, cell shape, and mesenchymal stem cell potency. Stem Cells 30(3): 548-560
  4. Chi, S. et al. (2016) A CRISPR-based toolbox for studying T cell signal transduction. Biomed Res Int 2016

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