Clone:
MJ4-27G12
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2b
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
ITGAX, CR4, SLEB6, 95, integrin αX, p150

Extended validation for CD11c Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with MJ4-27G12
S-HCL-3-
3.9-
Bu15-
B-ly6-
REA618-
Cells were incubated with an excess of purified unconjugated CD11c (MJ4-27G12) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11c (MJ4-27G12). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD11c (MJ4-27G12). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD11c (MJ4-27G12). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD11c Antibody, anti-human

Overview

This CD11 antibody reacts with human CD11c, a 145–150 kDa type I transmembrane glycoprotein, which is also known as integrin αX or CR4. It is expressed on monocytes, macrophages, NK cells, granulocytes, myeloid dendritic cells (MDCs), and subsets of T and B cells.
On myeloid dendritic cells, CD11c is highly expressed on type 1 myeloid dendritic cells (CD1c (BDCA-1)
+
CD123
low
MDC1s) and low on type 2 myeloid dendritic cells (CD1c (BDCA-1)
-
CD123
-
MDC2s).
CD11c, also known as integrin integrin αX or CR4, has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.

Alternative names

ITGAX, CR4, SLEB6, 95, integrin αX, p150

Detailed product information

Technical specifications

CloneMJ4-27G12
Clonalitymonoclonal
Isotypemouse IgG2b
Isotype controlIsotype Control Antibody, mouse IgG2b
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD11c
Alternative names of antigenITGAX, CR4, SLEB6, 95, integrin αX, p150
Molecular mass of antigen [kDa]126
Distribution of antigenB cells, T cells, NK cells, dendritic cells, granulocytes, monocytes, macrophages, leukemia cells, B cells, dendritic cells, granulocytes, lymphocytes, macrophages, monocytes, myeloid leukemia cells, NK cells, T cells, leukemia cells
Entrez Gene ID3687
RRIDAB_2726179, AB_2726457, AB_2726180, AB_2726453, AB_2726176, AB_2726460, AB_2726183, AB_2726458, AB_2726181, AB_2726454, AB_2726177, AB_2726459, AB_2726182, AB_2726455, AB_2726178, AB_2660150, AB_871586, AB_2726456

Resources for CD11c Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD11c Antibody, anti-human

Publications

  1. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
  2. Bendiss-Vermare, N. et al. (2001)
    Human thymus contains IFN-alpha-producing CD11c
    , myeloid CD11c
    +
    , and mature interdigitating dendritic cells.
    J. Clin. Invest. 107: 835-844
  3. Barclay, A. N. et al. (1997) In: The Leukocyte Antigen Facts Book, Academic Press, San Diego, CA. (2nd edition): 161-162

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