Clone:
REA792
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC, MC
Alternative names:
LAMP-1, LAMPA, LGP120, LAMP1

Extended validation for CD107a (LAMP-1) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA792
H4A3++
508921+
Cells were incubated with an excess of purified unconjugated CD107a (LAMP-1) (REA792) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD107a (LAMP-1). Splenocytes from C57BL/6 mice were stained with CD107a (LAMP-1) antibodies and with a suitable counterstaining. As a control, CD107a (LAMP-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD107a (LAMP-1). Splenocytes from C57BL/6 mice were stained with CD107a (LAMP-1) antibodies and with a suitable counterstaining. As a control, CD107a (LAMP-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD107a (LAMP-1). Splenocytes from C57BL/6 mice were stained with CD107a (LAMP-1) antibodies and with a suitable counterstaining. As a control, CD107a (LAMP-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD107a (LAMP-1). Splenocytes from C57BL/6 mice were stained with CD107a (LAMP-1) antibodies and with a suitable counterstaining. As a control, CD107a (LAMP-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD107a (LAMP-1). Splenocytes from C57BL/6 mice were stained with CD107a (LAMP-1) antibodies and with a suitable counterstaining. As a control, CD107a (LAMP-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD107a (LAMP-1) Antibody, anti-human, REAfinity™

Overview

Clone REA792 recognizes the CD107a antigen, also known as lysosome-associated membrane protein 1 (LAMP-1), a 110–140 kDa type I membrane glycoprotein. It is a widely expressed intracellular protein, located in the lysosomal/endosomal membrane. CD107a transiently located on the plasma membrane can be used as a marker for CD8
+
T cell degranulation following stimulation. It is also expressed to a lower extent on activated NK cells.
Additional information: Clone REA792 displays negligible binding to Fc receptors.

Alternative names

LAMP-1, LAMPA, LGP120, LAMP1

Detailed product information

Technical specifications

CloneREA792
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
pigtail monkey (
Macaca nemestrina
)
,
african green monkey (
Chlorocebus aethiops
)
, baboon,
chimpanzee (
Pan troglodytes
)
AntigenCD107a (LAMP-1)
Alternative names of antigenLAMP-1, LAMPA, LGP120, LAMP1
Molecular mass of antigen [kDa]42
Distribution of antigendendritic cells, epithelial cells, granulocytes, macrophages, T cells, neutrophils
Entrez Gene ID3916
RRIDAB_2654472, AB_2654473, AB_2654474, AB_2654475, AB_2654476, AB_2654477, AB_2654478, AB_2654479, AB_2654480, AB_2654481, AB_2654482, AB_2654483, AB_2654484, AB_2654485, AB_2654486, AB_2654487, AB_2654488, AB_2654489, AB_2654490, AB_2654491, AB_2654492, AB_2654493, AB_2654494, AB_2819666, AB_2654471

Resources for CD107a (LAMP-1) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD107a (LAMP-1) Antibody, anti-human, REAfinity™

Publications

  1. Fukuda, M. et al. (1988) Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences. J. Biol. Chem. 263(35): 18920-18928
  2. Mane, S. M. et al. (1989) Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem Biophys. 268(1): 360-378
  3. Betts, M. R. et al. (2003)
    Sensitive and viable identification of antigen-specific CD8
    +
    T cells by a flow cytometric assay for degranulation.
    J. Immunol. Methods 281: 65-78

Related products for
CD107a (LAMP-1) Antibody, anti-human, REAfinity™

4 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?