Are you looking for an easy, fast, and reliable method for extracting nuclei from tissue?
Our Nuclei Extraction Buffer enables the generation of high quality and high yield nuclei suspensions from a wide range of fresh and frozen tissues. The Nuclei Extraction Buffer works in combination with the gentleMACS™ Dissociators and C Tubes and is ideal for downstream molecular analysis
Regardless if fresh or frozen, high yields of nuclei can be obtained from samples using the Nuclei Extraction Buffer in combination with the gentleMACS Octo Dissociator.
No matter the tissue type, high numbers of nuclei can be extracted using the Nuclei Extraction Buffer. Here we show the high nuclei yields from dissociated snap frozen mouse liver, heart and kidney and from OCT embedded human tumors, including melanoma, breast cancer, colorectal cancer and prostatic cancer.
Single nuclei sequencing applications require high quality single nuclei suspensions. Performed in a cold environment, our method allows the obtention of intact nuclei with highly preserved RNA for optimal subsequent genomic applications.
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