Tumor cell isolation

Isolation of pure and viable tumor cells is a prerequisite for reliable cancer research. Tumor Cell Isolation Kits and suitable isolation protocols yield pure untouched cancer cells of different tumor entities. Subsequently, specific subpopulations can be isolated by positive separation, allowing for high-quality analysis of low-frequent tumor populations like cancer stem cells. 

Application protocols

Discover our different workflows protocols for untouched isolation of tumor cells from different starting material.

Application data by workflow step

Tumor storage and dissociation

Gentle tumor dissociation is a prerequisite for efficient downstream cell separation, analysis, or culture. gentleMACS™ Technology ensure rapid and standardized dissociation of tissue samples into single-cell suspensions.

Comparison of single cells yields from tissue stored using either MACS Tissue Storage Solution or competitor products
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Optimized storage of tumor tissue

Need to store freshly dissected tumor tissue? The MACS® Tissue storage solution enables storage of tissue for up to 48 hours without compromising viability or causing unwanted effects like cell activation or apoptosis.

Untouched tumor cell isolation

Depletion of non-tumor cells from dissociated tissue is essential for receiving pure tumor cells of interest. Based on comprehensive screenings on primary tumor material, cell lines, and health tissues, we have defined combinations of antibodies recognizing all cells of the tumor microenvironment but not the tumor cells. Using these antibodies coupled to MicroBeads can eliminate >95% of contaminating cells in less than 20 min.

Analysis of untouched CD133+ tumor cells after non-tumor cell depletion
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Rapid isolation of untouched human tumor cells

Depletion of non-tumor cells from dissociated primary tumors can be performed by magnetic cell separation using the Tumor Cell Isolation Kit (TDK), human.

As the isolated tumor cells are untouched, subsequent tumor subpopulations can be isolated by MACS® Technology as shown for the isolation of CD133+ cancer stem cells.

Isolation of untouched human tumor cells from PDX brain tumors after mouse cell depletion
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Untouched human tumor cells from PDX mouse models

The Mouse Cell Depletion Kit effectively removes post-dissociation debris and depletes infiltrating mouse cells from human GBM cells in PDX models.  This delivers untouched human tumor cells with a purity of 98%.  

Isolation of untouched mouse tumor cells from different tumor entities
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Untouched tumor cell isolation regardless of tumor entity

Using the Tumor Cell Isolation Kit, mouse untouched tumor cells can be isolated from bulk tumors. As the antibody cocktail was developed to deplete unwanted non-tumor cells, the isolation is independent of tumor-specific surface markers.

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A complete workflow for isolating pure tumor cell populations from primary tissue for improved downstream analysis

Listen to the lecture delivered by Dr. Olaf Hardt during Tri-Con 2016 at the Moscone North Conference Center, San Francisco, CA, and learn more about isolating pure tumor cell populations from primary tissue for improved downstream analyses using MACS® Technology.

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A revolutionary workflow for isolating pure cell populations from primary tumor tissue

Join Dr. Carsten Studte and his webinar on the isolation of cells from tumor tissues, including the optimized and automated dissociation of solid tissues.

Analysis of tumor populations

Depletion of unwanted cells from cell populations improves analysis as well as culture of target cells. Fast and easy magnetic separation using MACS MicroBeads allows for accurate downstream analysis avoiding bias caused by contaminating cells.

Exosome sequencing of tumor samples prior to and after mouse cell depletion
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Improved downstream analysis of untouched tumor cells

Depletion of contaminating mouse cells allows for accurate downstream analysis of human tumor xenografts by next generation sequencing. Untouched isolation by magnetic separation is easy and fast (<20 min) and can be used for all kinds of xenografted human material without the need for a positive marker expressed on human cells.

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