Miltenyi Biotec-tested panel 28 (MBTP 28)
This application protocol describes the flow cytometric analysis of virus-specific T cells after enrichment of IFN-γ+ cells from human blood. IFN-γ+ cells were enriched using the CliniMACS® Cytokine Capture System (IFN-gamma) to obtain viable single cell suspensions ready for downstream applications, such as flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|7-AAD Staining Solution||-||Viability||-||655–730 (blue)|
|CD4||REA623||T helper cells||VioGreen™||525/50 (violet)|
|CD3||REA613||T cells||FITC||525/50 (blue)|
|CD8||REA734||Cytotoxic T cells||APC-Vio® 770||750 LP (red)|
|IFN-γ||45-15||Activation marker||PE||585/40 (blue)|
|CD45RO||REA611||Memory T cells||PE-Vio 770||750 LP (blue)|
|CD62L||145/15||Naive and memory T cells||APC||655–730 (red)|
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