1. High consistency and reproducibility:
Recombinant antibodies are derived from a defined set of genes, and the production process is highly standardized. Therefore, these antibodies are consistent in their structure and performance, leading to high experimental reproducibility.
Figure: Human PBMCs from a single donor were stained with CD56 antibodies conjugated to FITC, PE, APC, APC-Vio770, or PE-Vio770 from two different production lots, and analyzed on the MACSQuant® Analyzer. The overlayed histograms represent the different lots. Tandem Signal Enhancer (# 130-099-888) was used to increase stain indices of tandem dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. In case of staining with CD56 conjugated to tandem dyes, DAPI was used to exclude dead cells from the analysis.