Clone:
Ter-119
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Applications:
FC, MICS, IF, IHC
Alternative names:
Ly76

Extended validation for Ter-119 Antibody, anti-mouse

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using Ter-119 (Ter-119). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using Ter-119 (Ter-119). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using Ter-119 (Ter-119). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for Ter-119 Antibody, anti-mouse

Overview

Clone Ter-119 recognizes the Ter-119 antigen which is expressed on mature erythrocytes and erythroid precursor cells in adult blood, spleen, and bone marrow, and in the embryonic yolk sac and fetal liver. The Anti-Ter-119 antibody does not react with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activity. In adult mice, Anti-Ter-119 reacts with 20–25% of bone marrow cells and approximately 50% of spleen cells, but not with thymocytes or lymph node cells.

Alternative names

Ly76

Detailed product information

Technical specifications

CloneTer-119
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenTer-119
Alternative names of antigenLy76
Distribution of antigenred blood cells
Entrez Gene ID104231
RRIDAB_2751385, AB_2733714, AB_2733715, AB_2784480, AB_2660083, AB_2660084, AB_2660085, AB_2905443, AB_2751397

Resources for Ter-119 Antibody, anti-mouse

Certificates

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Ter-119 Antibody, anti-mouse

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