Clone:
REA845
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
KLRF1, CLEC5C

Extended validation for NKp80 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA845
4A4.D10++
5D12-
Cells were incubated with an excess of purified unconjugated NKp80 (REA845) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using NKp80 (REA845). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using NKp80 (REA845). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using NKp80 (REA845). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using NKp80 (REA845). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using NKp80 (REA845). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for NKp80 Antibody, anti-human, REAfinity™

Overview

Clone REA845 recognizes the human NKp80 antigen, an 80 kDa homodimeric surface molecule belonging to the family of C-type lectin-like receptors. NKp80 is expressed on all human natural killer (NK) cells and on a small subset of effector memory CD8
+
T cells. NKp80 stimulates NK cell cytotoxicity and cytokine release and appears to co-operate with other triggering receptors to induce optimal NK cell activation upon interaction with potential target cells. The ligand of NKp80 is activation-induced C-type lectin (AICL), also called CLEC2B.
Additional information: Clone REA845 displays negligible binding to Fc receptors.

Alternative names

KLRF1, CLEC5C

Detailed product information

Technical specifications

CloneREA845
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
,
rhesus monkey (
Macaca mulatta
)
AntigenNKp80
Alternative names of antigenKLRF1, CLEC5C
Distribution of antigenNK cells, T cells
Entrez Gene ID51348
RRIDAB_2653020, AB_2653021, AB_2653022, AB_2653023, AB_2653024, AB_2653025, AB_2653026, AB_2653027, AB_2653028, AB_2653029, AB_2653030, AB_2653031, AB_2751105, AB_2751104, AB_2653032, AB_2653033, AB_2889721, AB_2653019

Resources for NKp80 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for NKp80 Antibody, anti-human, REAfinity™

Publications

  1. Kuttruff, S. et al. (2009) NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood 113: 358-369
  2. Welte, S. et al. (2006) Mutual activation of natural killer cells and monocytes mediated by NKp80-AICL interaction. Nat. Immunol. 7: 1334-1342
  3. Vitale, M. et al. (2001) Identification of NKp80, a novel triggering molecule expressed by human NK cells. Eur. J. Immunol. 31: 233-242

Related products for
NKp80 Antibody, anti-human, REAfinity™

3 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?