Clone:
4A4.D10
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC
Alternative names:
KLRF1, CLEC5C

Extended validation for NKp80 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 4A4.D10
REA845++
5D12-
Cells were incubated with an excess of purified unconjugated NKp80 (4A4.D10) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for NKp80. Human peripheral blood mononuclear cells (PBMCs) were stained with NKp80 antibodies and with a suitable counterstaining. As a control, NKp80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using NKp80 (4A4.D10). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using NKp80 (4A4.D10). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using NKp80 (4A4.D10). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for NKp80 Antibody, anti-human

Overview

NKp80 is an 80 kDa homodimeric surface molecule and belongs to the family of C-type lectin-like receptors. It is expressed on all human natural killer (NK) cells and a small subset of effector memory CD8
+
T cells. NKp80 stimulates NK cell cytotoxicity and cytokine release and appears to co-operate with other triggering receptors to induce optimal NK cell activation upon interaction with potential target cells. The ligand of NKp80 is AICL (activation-induced C-type lectin), also called CLEC2B.

Alternative names

KLRF1, CLEC5C

Detailed product information

Technical specifications

Clone4A4.D10
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenNKp80
Alternative names of antigenKLRF1, CLEC5C
Molecular mass of antigen [kDa]27
Distribution of antigenNK cells, T cells
Entrez Gene ID51348
RRIDAB_2857749, AB_2857813, AB_2857803, AB_2905351, AB_2905350, AB_10829948, AB_10829308, AB_10828905, AB_2857761

Resources for NKp80 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for NKp80 Antibody, anti-human

Publications

  1. Kuttruff, S. et al. (2009) NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood 113: 358-369
  2. Welte, S. et al. (2006) Mutual activation of natural killer cells and monocytes mediated by NKp80-AICL interaction. Nat. Immunol. 7: 1334-1342
  3. Vitale, M. et al. (2001) Identification of NKp80, a novel triggering molecule expressed by human NK cells. Eur. J. Immunol. 31: 233-242

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