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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Our local employees are always happy to answer your questions. Highly trained and experienced teams in your country can provide quick, helpful, and comprehensive support.
Miltenyi Biotec Ltd.
Almac House, Church Lane
Bisley, Surrey GU24 9DR
United Kingdom
Phone: +44 1483 799 800
Fax: +44 1483 799 811
E-Mail: macs@miltenyibiotec.co.uk
Web: www.miltenyibiotec.com
For immediate technical support, use our live chat. Connect with us
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population using the Mouse Cell Depletion Kit, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and gentleMACS Octo Dissociator. The figure shows the sample before separation and the isolated human tumor cells after the mouse cells have been depleted. The Labeling Check Reagent-APC was used to analyze mouse cells. |
Unseparated fraction | Isolated human tumor cells |
Mouse Cell Depletion KitFigure 1EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population using the Mouse Cell Depletion Kit, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and gentleMACS Octo Dissociator. The figure shows the sample before separation and the isolated human tumor cells after the mouse cells have been depleted. The Labeling Check Reagent-APC was used to analyze mouse cells. | Mouse Cell Depletion KitFigure 1EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population using the Mouse Cell Depletion Kit, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and gentleMACS Octo Dissociator. The figure shows the sample before separation and the isolated human tumor cells after the mouse cells have been depleted. The Labeling Check Reagent-APC was used to analyze mouse cells. |
EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population and subsequently, the unseparated fraction, the human tumor cell fraction, and the mouse cell containing fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for murine vimentin (red) and human EpCAM (green). Cell nuclei were stained with DAPI (blue). |
Unseparated fraction | Isolated human tumor cells |
Mouse Cell Depletion KitFigure 2EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population and subsequently, the unseparated fraction, the human tumor cell fraction, and the mouse cell containing fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for murine vimentin (red) and human EpCAM (green). Cell nuclei were stained with DAPI (blue). | Mouse Cell Depletion KitFigure 2EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population and subsequently, the unseparated fraction, the human tumor cell fraction, and the mouse cell containing fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for murine vimentin (red) and human EpCAM (green). Cell nuclei were stained with DAPI (blue). |
Mouse cell fraction | |
Mouse Cell Depletion KitFigure 2EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population and subsequently, the unseparated fraction, the human tumor cell fraction, and the mouse cell containing fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for murine vimentin (red) and human EpCAM (green). Cell nuclei were stained with DAPI (blue). |
EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population using the Mouse Cell Depletion Kit, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and gentleMACS Octo Dissociator. The figure shows the sample before separation and the isolated human tumor cells after the mouse cells have been depleted. The Labeling Check Reagent-APC was used to analyze mouse cells. |
Unseparated fraction | Isolated human tumor cells |
Mouse Cell Depletion KitFigure 1EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population using the Mouse Cell Depletion Kit, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and gentleMACS Octo Dissociator. The figure shows the sample before separation and the isolated human tumor cells after the mouse cells have been depleted. The Labeling Check Reagent-APC was used to analyze mouse cells. | Mouse Cell Depletion KitFigure 1EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population using the Mouse Cell Depletion Kit, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and gentleMACS Octo Dissociator. The figure shows the sample before separation and the isolated human tumor cells after the mouse cells have been depleted. The Labeling Check Reagent-APC was used to analyze mouse cells. |
EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population and subsequently, the unseparated fraction, the human tumor cell fraction, and the mouse cell containing fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for murine vimentin (red) and human EpCAM (green). Cell nuclei were stained with DAPI (blue). |
Unseparated fraction | Isolated human tumor cells |
Mouse Cell Depletion KitFigure 2EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population and subsequently, the unseparated fraction, the human tumor cell fraction, and the mouse cell containing fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for murine vimentin (red) and human EpCAM (green). Cell nuclei were stained with DAPI (blue). | Mouse Cell Depletion KitFigure 2EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population and subsequently, the unseparated fraction, the human tumor cell fraction, and the mouse cell containing fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for murine vimentin (red) and human EpCAM (green). Cell nuclei were stained with DAPI (blue). |
Mouse cell fraction | |
Mouse Cell Depletion KitFigure 2EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population and subsequently, the unseparated fraction, the human tumor cell fraction, and the mouse cell containing fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for murine vimentin (red) and human EpCAM (green). Cell nuclei were stained with DAPI (blue). |
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