Profiling cytokine-secreting cells and intracellular cytokines by flow cytometry

Characterize cellular effector functions and antigen-specific responses based on cytokine production at the single-cell level, even directly from whole blood or PBMCs. MACS® Cytokine Secretion Assays are unique tools for the detection of a wide range of cytokines secreted by viable cells, enabling highly sensitive analyses of target populations. For activated CD4+ and CD8+ T cells, Rapid Cytokine Inspector Kits are designed to support large-scale analyses with fast and reliable detection of intracellular cytokine production with a minimum number of workflow steps. Each Rapid Cytokine Inspector Kit includes everything for processing – from fixation and permeabilization to an optimized combination of antibodies – and delivers highly accurate and reproducible results.

Example protocols and tools for MACS Cytokine Secretion Assays

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Sensitive, high-throughput cytokine analysis in CAR T cell workflows

Rapid identification and enumeration of cytokine-producing T cells as well as detailed examination of newly engineered CAR T cells.

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