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Splenocytes from C57BL/6 mice, either left unlabeled (left image) or labeled with anti-mouse CD16/CD32 antibodies (isotype rat IgG2b), were stained with Anti-IgG2b antibodies as well as with CD45R (B220) antibodies. The FcR Blocking Reagent has been used to avoid Fc receptor-mediated antibody labeling. Flow cytometry was performed with the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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