CD85j (ILT2) antibodies, human

CD85j (ILT2) antibodies, human

Clone: GHI/75 | Dilution: 1:11
Clone GHI/75 reacts with CD85j (ILT2), an inhibitory immunoreceptor of the immunoglobulin-like transcript (ILT) family. CD85j is a type I transmembrane protein with four extracellular Ig-like domains and four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic tail. The inhibitory signal is transmitted by recruiting SH2-containing protein tyrosine phosphatase-1 that dephosphorylates and inactivates downstream tyrosine kinases. Expression of CD85j is found on B cells, monocytes, dendritic cells, and subsets of natural killer (NK) and T cells. CD85j is known to bind classical and non-classical MHC class I molecules, human cytomegalovirus UL18 protein, and some HLA-C alleles, as ligands. Crosslinking of CD85j prevents activation of B cells, T cells, NK cells, macrophages, and downregulation of effector mechanisms, such as NK and T cells cytolytic functions.

Alternative names

LILRB1, ILT2, LIR-1, MIR-7

Technical specifications

  • Antigen: CD85j (ILT2)
  • Clone: GHI/75
  • Isotype: mouse IgG2bκ
  • Isotype control: Mouse IgG2b – isotype control antibodies
  • Alternative names of antigen: LILRB1, ILT2, LIR-1, MIR-7
  • Entrez Gene ID: 10859
  • Molecular mass of antigen [kDa]: 68
  • Distribution of antigen: B cells, dendritic cells, leukemia cells, lymphocytes, monocytes, myeloid cells, NK cells, plasma cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, PE-Vio770, Biotin, FITC
  • Selected references

    1. Borges, L. et al. (1997) A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules. J. Immunol. 159: 5192-5196
    2. Morel, E. and Bellón, T. (2008) HLA Class I molecules regulate IFN-{gamma} production induced in NK cells by target cells, viral products, or immature dendritic cells through the inhibitory receptor ILT2/CD85j. J. Immunol. 181: 2368-2381
    3. Merlo, A. et al. (2005) Inhibitory receptors CD85j, LAIR-1, and CD152 down-regulate immunoglobulin and cytokine production by human B lymphocytes. Clin. Diagn. Lab. Immunol. 12: 705-712
Product options: 5
for 100
tests
(1)
GBP 150.00
for 100
tests
(1)
GBP 175.00
for 30
tests
(1)
GBP 80.00
for 100
tests
(1)
GBP 190.00
for 100
tests
(1)
GBP 150.00

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