Find the products and resources you are looking for!
Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Mouse dendritic cells (DCs) were specifically activated in C57/Bl6 mice by subcutaneous injection of 50 μL of CD40 (1 mg/0.5 mL) or an isotype control (left images) into the left and right footpad. DC activation was analyzed in popliteal fossa lymph nodes after 72 hours. Cells were stained with CD11c-FITC, CD86-PE, CD205-Biotin/Anti-Biotin-APC, and CD3ε-PerCP antibodies, and analyzed by flow cytometry. The FcR Blocking Reagent was used to avoid Fc receptor–mediated antibody labeling. Cell debris, dead cells, and CD3+
T cells were excluded from the analysis based on scatter signals as well as propidium iodide and CD3ε-PerCP fluorescence.
Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.