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Human peripheral blood mononuclear cells (PBMCs) were stained with CD38 antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD14 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones.
|Other clones||Overlap in epitope recognition with REA572|
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