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Human peripheral blood mononuclear cells (PBMCs) were stained with CD279 (PD1) antibodies as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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