CD234 (DARC) antibodies, human

CD234 (DARC) antibodies, human

Clone: REA376 | Dilution: 1:11
Clone REA376 recognizes the human CD234 antigen, a multi-pass membrane protein also known as duffy antigen receptor for chemokines (DARC) or Fy glycoprotein (FY). CD234 is responsible for the duffy blood group system and belongs to a family of silent seven-transmembrane spanning receptors, which do not couple to G-proteins and therefore do not transmit intracellular signals like other G-protein–coupled chemokine-receptors leading to integrin activation or cell motility. It binds to a broad range of inflammatory chemokines of both the CC and the CXC chemokine families, but does not interact with homeostatic chemokines. CD234 is expressed on erythrocytes, cerebellar neurons on postcapillary venule, and capillary endothelial cells in a number of peripheral organs including lymph nodes, the lung, and the kidney. In these organs, endothelial CD234 is specifically expressed in the vascular segment involved in leucocyte extravasation. CD234 was shown to mediate chemokine transcytosis from the basolateral to the luminal side of the endothelium, where the chemokines remain immobilized on the tips of endothelial apical microvilli and enhance leucocyte trafficking
in vivo
Additional information: Clone REA376 displays negligible binding to Fc receptors.

Alternative names


Technical specifications

  • Antigen: CD234 (DARC)
  • Clone: REA376
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: ACKR1, CCBP1, WBCQ1, DARC, Dfy, FY, GPD, GpFy
  • Entrez Gene ID: 2532
  • Molecular mass of antigen [kDa]: 36
  • Distribution of antigen: red blood cells, neurons, lung, lymph node, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, PE-Vio770
  • Selected references

    1. Chaudhuri, A. et al. (1993)
      Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the
      Plasmodium vivax
      malaria parasite.
      Proc. Natl. Acad. Sci. U.S.A. 90(22): 10793-10797
    2. Graham, G. J. et al. (2012) The biochemistry and biology of the atypical chemokine receptors. Immunol. Lett. 145(1-2): 30-38
    3. Minten, C. et al. (2014) DARC shuttles inflammatory chemokines across the blood-brain barrier during autoimmune central nervous system inflammation. Brain 137(5): 1454-1469
Product options: 2
for 30
GBP 80.00
for 100
GBP 195.00

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