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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with CD3 and CD28 antibodies for 3 days. Cells were then stained with CD223 antibodies as well as with CD8 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
In order to compare the epitope specificity of REAfinity Clone REA351 with other known clones, a competition assay was performed.
Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
|Other clones||Overlap in epitope recognition with REA351|
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