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Mouse spleen cells (BALB/c) were stained with CD21/CD35 antibodies as well as with CD19-APC or CD19-PE and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of CD21/CD35-PE-Vio770.
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