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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
A single-cell suspension was prepared using the Epidermis Dissociation Kit. Cells were stained with CD207 (Langerin) antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), or PE-Vio770 (E) as well as with Anti-MHC Class II antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cells labeled with CD207 (Langerin)- Biotin (F) were stained with Anti-Biotin-PE in addition. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of CD207(Langerin)-PE-Vio770.
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