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IFN-α stimulated human peripheral blood mononuclear cells (PBMCs) (day-2) were stained with CD169 (Siglec-1) antibodies as well as with CD14 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and either propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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