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BALB/c mouse spleen cells were activated with plate-bound CD3 and soluble CD28 for 24 hours and were stained with CD153 antibodies as well as with CD4-FITC and CD45R (B220)-PerCP and analyzed by flow cytometry using the MACSQuant®
Analyzer. The unstimulated controls can be seen in the left images. Cell debris, dead cells and CD45R (B220)+
cells were excluded from the analysis based on scatter signals and propidium iodide and PerCP fluorescences.
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