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CD3/CD28-activated BALB/c mice spleen cells were stained with CD152 antibodies conjugated to PE (A), APC (B) and Biotin (C) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD152-Biotin were stained with Anti-Biotin-PE in addition. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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