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Thymocytes from C57BL/6 mice were stained with CD4 and CD8a antibodies and fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with Anti-TOX antibodies or with the corresponding REA Control (I) antibodies (left peak) and analyzed by flow cytometry using the MACSQuant®
thymocytes are shown in the histogram. Cell debris were excluded from the analysis based on scatter signals.
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