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U937 cells, either left unstimulated (left peak) or stimulated with 1000 U/mL human IFN-γ for 15 minutes at 37 °C, were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were then stained with Anti-Stat1 pY701 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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