Clone REA1219 recognizes the extracellular domain of human Notch3, one of four members of the Notch family of receptors. Upon binding its membrane-bound ligand of the Delta-like or Jagged family members, the Notch receptor undergoes two proteolytic cleavage steps eventually releasing the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, where it activates transcription within a multi-protein complex. Notch3 is expressed on various various hematopetic cells, including T cell precursors in the thymus and activated peripheral T cells. It can also be expressed on vascular smooth muscle and cells of the central nervous system. In addition, the altered expression of Notch3 by various leukemias, lymphomas and cancers has been reported.
Additional information: Clone REA1219 displays negligible binding to Fc receptors.