Anti-Notch1 antibodies, mouse

Anti-Notch1 antibodies, mouse

Clone: 22E5 | Dilution: 1:10
Notch1 is a 300 kDa member of the Notch family of type I transmembrane glycoproteins. Following cleavage in the Golgi, Notch forms a heterodimer composed of a 180-200 kDa disulfide-linked extracellular domain, and a 120 kDa membrane-bound intracellular portion. Upon ligand binding the latter is cleaved to form the notch intracellular domain transcription factor. Notch signaling controls cell fate decisions and is involved in multiple cellular differentiation steps during embryonic development and in adult organisms. In the immune system, Notch1 plays a non-redundant role for thymic T cell development
. Notch1 is also expressed by peripheral T cells and upregulated upon T cell activation
. The 22E5 antibody reacts with the extracellular part of the murine Notch1 protein.

Alternative names

Mis6, N1, TAN-1, lin-12

Technical specifications

  • Antigen: Notch1
  • Clone: 22E5
  • Isotype: rat IgG2aκ
  • Isotype control: Rat IgG2a – isotype control antibodies
  • Alternative names of antigen: Mis6, N1, TAN-1, lin-12
  • Entrez Gene ID: 18128
  • Molecular mass of antigen [kDa]: 269
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, Biotin, PE
  • Selected references

    1. Fiorini, E. et al. (2009) Dynamic regulation of notch 1 and notch 2 surface expression during T cell development and activation revealed by novel monoclonal antibodies. J. Immunol. 183(11): 7212-7222
    2. Radtke, F. et al. (2010) Notch signaling in the immune system. Immunity 32(1): 14-27
Product options: 3
30 µg in 1 mL (1)
GBP 50.00
30 µg in 1 mL (1)
GBP 50.00
30 µg in 1 mL (1)
GBP 30.00

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