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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left peak) or stimulated with CD3/ CD28 antibodies for 72 hours. Cells were then fixed and permeabilized using the Cell Signaling Buffer Set A, stained with Anti-Mcl-1 pS159 antibodies, and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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