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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
T cells were enriched with the CD4+
T Cell Isolation Kit II, mouse from splenocytes of BALB/c mice. Cells were stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence recombinant IL-4, IL-2, TGFß-1 and neutralizing Anti-IL-6 antibodies, Anti- IFN-γ antibodies and Anti-IL-12 antibodies for 5 days. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-9 antibodies or with the REA Control antibodies (left image), as well as with CD4 antibodies. Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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