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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 100 ng/mL LPS for 2 hours followed by incubation with 1 µg/mL brefeldin A for 4 hours. Cells were fixed, permeabilized, and intracellularly stained with Anti-IL-6 antibodies as well as with CD14 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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