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BALB/c spleen cells were stained with Anti-IgD antibodies, as well as with CD19 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cells labeled with Anti-IgD-Biotin were stained with Anti-Biotin-PE in addition. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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