Anti-HLA-DM antibodies, human

Anti-HLA-DM antibodies, human

Clone: REA406 | Dilution: 1:50
Clone REA406 recognizes the human HLA-DM antigen, a non-classical MHC class II molecule (MHC II) chaperone that works in lysosomes and endosomes. MHC II molecules are transmembrane glycoproteins which function by binding processed antigenic peptides and presenting them to CD4
+
T lymphocytes to initiate or maintain a specific immune response. Constitutive MHC II expression is restricted to professional antigen presenting cells including dendritic cells (DCs), B cells, and macrophages and to the epithelial cells of the thymus. Functional class II expression is highly dependent on the expression of two main chaperones, the invariant chain (Ii) and DM. Newly synthesized class II α- and β-chains form a nonameric complex with three Ii molecules in the endoplasmic reticulum where Ii blocks the peptide-binding groove and guides class II dimers to the endocytic pathway where Ii is proteolytically cleaved leaving the CLIP fragment bound to class II. HLA-DM, an endocytic chaperone, protects empty class II dimers until they bind a peptide capable to confer enough stability to the complex.
Additional information: Clone REA406 displays negligible binding to Fc receptors.

Alternative names

HLA-DMA, HLA-DMB, DMA, HLADM, RING6, D6S221E, RING7

Technical specifications

  • Antigen: HLA-DM
  • Clone: REA406
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: HLA-DMA, HLA-DMB, DMA, HLADM, RING6, D6S221E, RING7
  • Entrez Gene ID: 3108, 3109
  • Molecular mass of antigen [kDa]: 53
  • Distribution of antigen: dendritic cells, B cells, macrophages, thymic epithelial cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected references

    1. Oldford, S. A. et al. (2006) Tumor cell expression of HLA-DM associates with a Tʜ1 profile and predicts improved survival in breast carcinoma patients. Int. Immunol. 18(11): 1591-1602
    2. van Lith, M. et al. (2010) HLA-DP, HLA-DQ, and HLA-DR have different requirements for invariant chain and HLA-DM. J. Biol. Chem. 285(52): 40800-40808
    3. Pos, W. et al. (2013) Mechanisms of peptide repertoire selection by HLA-DM. Trends Immunol. 34(10): 495-501
Product options: 2
for 30
tests
(1)
GBP 70.00
for 100
tests
(1)
GBP 175.00