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T cells, isolated from mouse splenocytes, were either left unstimulated (left images) or stimulated with CD3ε/CD28/IL-2/IL-4 for 2 days and stained with CD4 antibodies. Cells were then fixed, permeabilized using the FoxP3 Staining Buffer Set, and stained with Anti-GATA3 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals.
In order to compare the epitope specificity of REAfinity Clone REA174 with other known clones, a competition assay was performed.
Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
|Other clones||Overlap in epitope recognition with REA174|
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