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A mixture of Hela cells and human peripheral blood mononuclear cells (PBMCs) were fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with Anti-EGR-1 antibodies or with the corresponding REA Control (I) antibodies (left peak) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris was excluded from the analysis based on scatter signals.
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