StraightFrom® Buffy Coat CD56 MicroBead Kit, human

Buffy Coat CD56 MicroBead Kit
, human

The StraightFrom
Buffy Coat CD56 MicroBead Kit has been developed for the rapid positive selection of CD56
cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat.

Background information

The CD56 antigen is expressed by most NK cells and a small subset of T cells. Upon activation of NK cells, the surface expression of CD56 is increased.

Detailed separation procedure

One entire buffy coat is labeled with StraightFrom
Buffy Coat CD56 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
Separator. After removing the column from the magnetic field, the magnetically retained CD56
cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD56
cells are well suited for further flow cytometric, functional, or molecular analysis.

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Product options: 1
for 1
buffy coat
GBP 445.00

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