Cell isolation directly from buffy coat

  • Isolation of highly pure cells straight from buffy coat in 30 minutes
  • No density gradient centrifugation, no erythrocyte lysis
  • Ready-to-use kits and fast protocols for manual or automated cell isolations

StraightFrom® Buffy Coat MicroBeads allow for the magnetic cell isolation directly from buffy coat. Leukocyte subsets such as CD4+, CD8+, CD14+, CD19+, or CD56+ cells can be purified using either MultiMACS™ Instruments or manual separators. Pre-enrichment of white blood cells, e.g. by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.

Buffy coat as starting material

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As by-products from whole blood donations, buffy coats contain the same amount of leukocytes as the original blood donation, but in higher concentrations. Buffy coats are ideally suited for enrichment of high amounts of leukocyte subset from PBMCs.

Manual cell separation

Manual isolation of pure cells directly from an entire buffy coat.
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With StraightFrom Buffy Coat MicroBead Kits, an entire buffy coat can be processed into target cells in less than 30 minutes.

Together with Whole Blood Columns (included in the kit) and magnetic separators such as the QuadroMACS™ Separator,  leukocytes can be manually enriched. First, the MicroBeads are added directly to the blood sample without any prior cell counting or PBMC isolation. Leukocytes can then be isolated straightforward within minutes without the need for density gradient centrifugation.

Find a product list of all StraightFrom Buffy Coat MicroBead Kits at the end of this page.

Automated cell isolation with the MultiMACS Cell24 Separator Plus

Semi-automated cell isolation directly from buffy coat with the MultiMACS™ Cell24 Separator Plus.
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The MultiMACS Cell24 Separator Plus is ideally suited to process an entire buffy coat in a single run with StraightFrom Buffy Coat MicroBeads. The 30-minute isolation procedure results in cell populations with high purities and recovery rates. With 12 Whole Blood Columns (included in the kit), the instrument allows for time saving cell isolation when processing large sample volumes, while providing reliability and reproducibility in day-to-day cell separations. 

For large-scale cell separations, the MultiMACS X  is the instrument of choice.
 


 

Data

Separation of CD4+ cells using StraightFrom® Buffy Coat CD4 MicroBeads

CD4+ cells can be isolated straight from a buffy coat using the StraightFrom® Buffy Coat CD4 MicroBead Kit, human and the MultiMACS™ Cell24 Separator Plus.

Before separation
After separation

Separation of a buffy coat sample using the StraightFrom® Buffy Coat CD4 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD4-PE, CD14-APC, as well as CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells were triggered via CD45-VioBlue, cell debris, and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Videos

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Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products using the MultiMACS™ Cell24 Separator Plus.

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Webinar

Immune cell isolation without density gradient centrifugation

Learn from this webinar how to isolate immune cells straight from blood or blood products without density gradient centrifugation.

 

 

Resources

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Brochure

StraightFrom MicroBeads - Cell isolation directly from blood products

Download the brochure to get all the details on our StraightFrom MicroBead Technology.

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Scientific poster

Isolation of functional cells straight from buffy coat, LRSC, and Leukopak®

Maike Schlemminger, Silke Weber-Lohmann, Martin Kolnik*, Zhong Yu, Susanne Höher-Peters, and Gregor Winkels

Miltenyi Biotec, Bergisch Gladbach, Germany

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