REAlease® MicroBeads

Magnetic cell isolation with reversible cell labeling

  • Bead-free cells suited for second positive selection
  • Label-free cells for any downstream application 
  • Recombinant antibody fragments for reproducible results

REAlease Technology

REAlease Technology allows for magnetic cell isolation and subsequent removal of any beads and labels from the cells in just a few simple steps. The technology relies on recombinantly engineered antibody fragments instead of whole antibodies for cell surface labeling. Monomeric fragments have a low affinity for cell surface markers. However, when multimerized as part of the REAlease Complex, the fragments bind markers with high avidity. REAlease MicroBeads bound to the REAlease Complex facilitate cell isolation using MACS® Columns and Separators. Disruption of the complex by adding REAlease Release Reagent after cell isolation leads to monomerization and spontaneous dissociation of the antibody fragments from the cell surface, resulting in label-free cells.

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New opportunities in cell separation
REAlease Technology is an indirect cell labeling method for positive selection of target cells. The REAlease Biotin Complex binds to the target cells. Labeling of the REAlease Biotin Complex with Anti-Biotin MicroBeads allows for magnetic isolation of these cells. Following cell separation, both MicroBeads and REAlease Biotin Complex are gently removed, leaving the cells bead-and label-free. Enriched cells are then suitable for magnetic re-labeling and any application where label-free cells are essential.


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REAlease Immunomagnetic Separation Technology

Reversible cell labeling for maximal flexibility

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Scientific poster
REAlease Immunomagnetic Separation Technology with reversible labeling for positive selection of leukocytes

Philipp Steinbrueck, Zhong Yu, Ursula Bissa, Jennifer Brieden, and Christian Dose
Miltenyi Biotec GmbH, Bergisch Gladbach, Germany

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