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The iNKT cells were isolated from human PBMCs using Anti-iNKT MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-iNKT-PE, CD3-APC, and CD45-FITC and analyzed using the MACSQuant® Analyzer. Gating was performed according to CD45 expression and side scatter properties of the cells.
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